FangpanProfessor

My researchinterestsfocus ondevelopingstate of the artmass spectrometry technologies for the analysis ofglycoproteins. I have made great breakthroughs in qualitative and quantitative N-glycoproteomics.I further studied the roles of proteinglycosylationin neurodegenerative diseases.NowI’m working ondrug discovery and development by proteome-wide orthogonal approaches.In the future, I will focus on the drug discovery targeting glycosylation and glycoprotein biomarker for neurological disorders.

1. Supervisor of a master student for the lab rotation.“Quantitative proteomic and phosphoproteomic analysis of ovarian follicles under luteinizing hormone treatment”,2months, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany (2020.3-2020.4)

2. Guest lecturer, graduate course, “Sequence analysis of protein post-translational modifications by ESI-MS”, 2 hours, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany (2019.10-2019.12)

3. Teaching assistant, undergraduate class, “Analytical chemistry experiments”, ten classes, Fudan University (2012.3-2012.7)

4. Guest lecturer, undergraduate class,"Biochemistry", two classes, Soochow University

1. Pan Fang;Yanlong Ji;Ivan Silbern;Rosa Viner;Thomas Oellerich;Kuan-Ting Pan;Henning Urlaub*; Evaluation and Optimization of High-Field Asymmetric Waveform Ion Mobility Spectrometry for Multiplexed Quantitative Site-specific N-glycoproteomics,Analytical chemistry,2021.93(25),8846-8855.

2. Ivan Silbern;Pan Fang;Yanlong Ji; Lenz Christof; Henning Urlaub;Kuan-Ting Pan;Relative Quantification of Phosphorylated and Glycosylated Peptides from the Same Sample Using Isobaric Chemical Labelling with a Two-Step Enrichment Strategy,Quantitative Methods in Proteomics. Methods in Molecular Biology,2021, 2228. Humana, New York, NY.

3. Marie Wensien^#;Fabian Rabe von Pappenheim^#;Lisa-Marie Funk;Patrick Kloskowski;Ute Curth;Ulf Diederichsen;Jon Uranga;Jin Ye;Pan Fang;Kuan-Ting Pan;Henning Urlaub;Ricardo A. Mata;Viktor Sautner;Kai Tittmann*;A lysine-cysteine redox switch with an NOS bridge regulates enzyme function.Nature.2021,593(7859):460-464.

4.Pan Fang^#;Yanlong Ji^#; Ivan Silbern; Carmen Doebele; Momchil Ninov;Christof Lenz; Thomas Oellerich; Kuan-Ting Pan*; Henning Urlaub*; Astreamlined pipeline for multiplexed quantitative site-specic Nglycoproteomics,Nature communications, 2020,11(1): 5268.

5. Tina Ukmar-Godec^#;Pan Fang^#; Alain Ibáñez de Opakua^#; Fabian Henneberg; Aljaz Godec; Kuan-Ting Pan; M.-S. Cima-Omori; Ashwin Chari; Eckhard Mandelkow; Henning Urlaub; Markus Zweckstetter*; Proteasomal degradation of the intrinsically disordered protein tau at single-residue resolution,Science Advances,2020, 6(30): eaba3916.

6.Pan Fang^#;JuanJuan Xie^#; Shaoming Sang; Lei Zhang; Mingqi Liu; LujieYang; Yiteng Xu; Guoquan Yan; Jun Yao; Xing Gao; Wenjing Qian; Zhongfeng Wang; Yang Zhang*; Pengyuan Yang*; Huali Shen*; Multilayered N-Glycoproteome Proling Reveals Highly Heterogeneous and Dysregulated Protein N-Glycosylation Related to Alzheimer’s Disease,Analytical chemistry,2020, 92(1): 867-874.

7. Yu Xue; Juanjuan Xie;Pan Fang; Jun Yao; Guoquan Yan; Huali Shen*; Pengyuan Yang*; Study on behaviors and performances of universal Nglycopeptide enrichment methods,Analyst,2018, 143(8): 1870-1880.

8. Ming-Qi Liu^#; Wen-Feng Zeng^#;Pan Fang^#; Wei-Qian Cao^#; Chao Liu^#; GuoQuan Yan; Yang Zhang; Chao Peng; Jian-Qiang Wu; Xiao-Jin Zhang; Hui-Jun Tu; Hao Chi; Rui-Xiang Sun; Yong Cao; Meng-Qiu Dong; Bi-Yun Jiang; Jiang-Ming Huang; Hua-Li Shen; Catherine C.L. Wong*; Si-Min He*; Peng-Yuan Yang*; pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identication,Nature communications,2017, 8: 438.

9.Pan Fang; Xin-jian Wang; Yu Xue; Ming-qi Liu; Wen-feng Zeng; Yang Zhang; Lei Zhang; Xing Gao; Guo-quan Yan; Jun Yao; Hua-li Shen*; Peng-yuan Yang*; In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins,Oncotarget,2016, 7(25): 38796-38809.

10. Wen-Feng Zeng^#; Ming-Qi Liu^#; Yang Zhang; Jian-Qiang Wu;Pan Fang; Chao Peng; Aiying Nie; Guoquan Yan; Weiqian Cao; Chao Liu; Hao Chi; RuiXiang Sun; Catherine C. L. Wong*; Si-Min He*; Pengyuan Yang*; pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD- and CID-MS/MS and MS3,Scientific reports,2016, 6: 25102.

11.Pan Fang; Mingqi Liu; Yu Xue; Jun Yao; Yang Zhang; Huali Shen*; Pengyuan Yang*; Controlling nonspecic trypsin cleavages in LC-MS/MS-based shotgun proteomics using optimized experimental conditions,Analyst,2015, 140(22): 7613-7621.

12. Hong Li; Huali Shen; Guoquan Yan; Yang Zhang; Mingqi Liu;Pan Fang; Hongxiu Yu*; Pengyun Yang*; Site-specific structural characterization of Oglycosylation and identification of phosphorylation sites of recombinant osteopontin,BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2015, 1854(6): 581-591.

13. Hong Li; Liming Wei*;Pan Fang; Pengyuan Yang*; Recent advances in the fabrication and detection of lectin microarrays and their application in glycobiology analysis,Analytical Methods,2014, 6(7): 2003-2014.

1. Manfred Eigen Fellowship for postdoctoral fellows in Max Planck Institute for Biophysical Chemistry (2017.09–2020.01)

2. National Scholarship for Distinguished Doctoral Student (2015–2016).