Guoqiang Xu, Ph.D.
Professor
Department of Pharmacology
Email: gux2002@suda.edu.cn
Biography
Education
1991-1996 B.S. Fudan University, Shanghai, China
1996-1999 M.S. Fudan University, Shanghai, China
1999-2002 Ph.D. The University of Akron, Akron, OH, USA
Work experience
2003-2005 Postdoctoral Associate, Department of Chemistry and Chemical Biology, Cornell University, NY, USA
2006-2013 Postdoctoral Associate and Assistant Research Professor, Department of Pharmacology, Weill Cornell Medical College, NY, USA
2013-present Professor, Soochow Univesity, Department of Pharmacology, Jiangsu, China
Research Interests
Quantitative Proteomics
Post-translational Modifications and Biological Function
Mechanism of Drug Action
Research Fund
1. National Natural Science Foundation of China. Study on the mechanism of the apoptosis of gastric cancer cells mediated by DCAF15, a substrate receptor of a ubiquitin ligase, and targeted drug discovery (2022-2025).
2. National Natural Science Foundation of China. Mechanistic study of the modulation of UFBP1, the key component of the ufmylation system, on the sensitivity of gastric cancer cells to platinum-based chemotherapeutic drugs (2020-2023).
3. National Natural Science Foundation of China. Mechanistic study of the ubiquitin ligase substrate receptor cereblon and its mutant on neuronal development (2017-2020).
4. National Natural Science Foundation of China. Mechanistic study of the ADAM12 proteolytic function on the proliferation and metastasis of small cell lung cancer (2015-2018).
5. National Natural Science Foundation of China. Molecular mechanism of lenalidomide in the treatment of multiple myeloma. National Natural Science Foundation of China (2013-2016).
6. National Key R&D Program of China (2019-2024, 2018-2021).
7. National Basic Research Program of China (973 Program) (2013-2016).
Select Publications
1．Hu Z, Wang X, Li D, Cao L, Cui H,Xu G*. UFBP1, a key component in ufmylation, enhances drug sensitivity by promoting proteasomal degradation of oxidative stress-response transcription factor Nrf2.Oncogene2021, 40, 647-662.
2．Zhou L,Xu G*. The ubiquitination-dependent and -independent functions of cereblon in cancer and neurological diseases.J Mol Biol.2022, 434(5), 167457.
3．Lu J, Jiang H, Li D, Chen T, Wang Y, Pu Z*,Xu G*. Proximity labeling, quantitative proteomics, and biochemical studies revealed the molecular mechanism for the inhibitory effect of indisulam on the proliferation of gastric cancer cells.J. Proteome Res.2021, 20(9), 4462-4474.
4．Zhou L*, Huang X, Niesvizky R, Pu Z,Xu G*. Caspase-8 regulates the anti-myeloma activity of bortezomib and lenalidomide.J Pharmacol Exp Ther,2021,379(3):303-309.
5．Zhang Z, Cheng X, Jiang H, Gu J, Yin Y, Shen Z, Xu C, Pu Z, Li J-B*,Xu G*. Quantitative proteomic analysis of glycosylated proteins enriched from urine samples with magnetic ConA nanoparticles identifies potential biomarkers for small cell lung cancer.J Pharm Biomed Anal,2021, 206, 114352.
6．Kang N, Zou Y, Liang Q, Wang Y, Liu Y,Xu G, Fan H, Xu Q*, Yang S, Yu D. Anemoside B4 inhibits enterovirus 71 propagation in mice through upregulating 14-3-3 expression and type I interferon responses.Acta Pharmacol Sin.2022.43(4):977-991.
7．Zhang Y, Zha Z, Shen W, Li D, Kang N, Chen Z, Liu Y,Xu G*, Xu Q* Anemoside B4 ameliorates TNBS‑induced colitis through S100A9/MAPK/NF‑κB signaling pathway.Chin. Med.2021, 16(1), 11.
8．Hu Z, Li H, Wang X, Ullah K,Xu G*. Proteomic approaches for the profiling of ubiquitylation events and their applications in drug discovery.J. Proteomics2021, 231, 1039966 (Review).
9．Yu W, Wang B, Zhou L*,Xu G*. Endoplasmic reticulum stress-mediated p62 downregulation inhibits apoptosis via c-Jun upregulation.Biomol. Ther.2021, 29(2), 195-204.
10．Zhou L, Yu W, Jayabalan DS, Niesvizky R, Jaffrey SR, Huang X*,Xu G*. Caspase-8 inhibition prevents the cleavage and degradation of E3 ligase substrate receptor cereblon and potentiates its biological function.Front. Cell Dev. Biol.2020, 8, 605989.
11．Ullah K, Chen S, Lu J, Wang X, Liu Q, Zhang Y, Long Y, Hu Z*,Xu G*. The E3 ubiquitin ligase STUB1 attenuates cell senescence by promoting the ubiquitination and degradation of the core circadian regulator BMAL1.J. Biol. Chem.2020, 295(14), 4696-4708.
12．Yuan Y#, Miao Y#, Qian L, Zhang Y, Liu C, Liu J, Zuo Y, Feng Q, Guo T, Zhang L, Chen X, Jin L, Huang F, Zhang H, Zhang W, Li W,Xu G, Zheng H*. Targeting UBE4A revives viperin protein in epithelium to enhance host antiviral defense.Mol. Cell2020, 77(4), 734-747.e7.
13．Duan Q, Li D, Xiong L, Chang Z,Xu G*. SILAC quantitative proteomics and biochemical analyses reveal a novel molecular mechanism by which ADAM12S promotes the proliferation, migration, and invasion of small cell lung cancer cells through upregulating hexokinase 1.J. Proteome Res.2019,18, 2903-2914.
14．Zhou L,Xu G*. Cereblon attenuates DNA damage-induced apoptosis by regulating the transcription-independent function of p53.Cell Death Dis.2019,10, 69.
15．Ullah K, Zubia E, Narayan M, Yang J,Xu G*. Diverse roles of the E2/E3 hybrid enzyme UBE2O in the regulation of protein ubiquitination, cellular functions, and disease onset.FEBS J.2019,286, 2018-2034 (Review,Cover).
16．Chen S, Yang J, Yang L, Zhang Y, Zhou L, Liu Q, Duan C, Mieres CA, Zhou G*,Xu G*. Ubiquitin ligase TRAF2 attenuates the transcriptional activity of the core clock protein BMAL1 and affects the maximalPer1mRNA level of the circadian clock in cells.FEBS J.2018,285, 2987-3001 (Editor’s Choice).
17．Chen S#, Yang J#, Zhang Y, Duan C, Liu Q, Huang Z, Xu Y, Zhou L*,Xu G*. Ubiquitin-conjugating enzyme UBE2O regulates cellular clock function by promoting the degradation of the transcription factor BMAL1.J. Biol. Chem.2018,293, 11296-11309 (Cover).
18．Yang J#, Huang M#, Zhou L, He X, Jiang X, Zhang Y,Xu G*. Cereblon suppresses lipopolysaccharide-induced inflammatory response through promoting the ubiquitination and degradation of c-Jun.J. Biol. Chem.2018,293, 10141-10157.
19．Xiong L#, Yan W#, Zubia E#, Zhou Y, Zhang Y, Duan Q, Narayan M,Xu G*. Quantitative proteomics and biochemical analyses reveal the role of endoplasmin in the regulation of the expression and secretion of A Disintegrin And Metalloproteinase 12.J. Proteomics.2018,182, 34-44.
20．Zhang Y#, Duan C#, Yang J, Chen S, Liu Q, Zhou L, Huang Z, Xu Y,Xu G*.Deubiquitinating enzyme USP9X regulates cellular clock function by modulating the ubiquitination and degradation of a core circadian protein BMAL1.Biochem. J.2018,475, 1507-1522.
21．Zhu Y#, Lei Q#, Li D, Zhang Y, Jiang X, Hu Z,Xu G*. Proteomic and biochemical analyses reveal a novel mechanism for promoting protein ubiquitination and degradation by UFBP1, a key component of ufmylation.J. Proteome Res.2018,17, 1509-1520.
22．Zhou L, Hao Z, Wang G,Xu G*. Cereblon suppresses the formation of pathogenic protein aggregates in a p62-dependent manner.Hum. Mol. Genet.2018,27, 667-678.
23．Wang C, Li J, Takemaru KI, Jiang X,Xu G, Wang B*. Centrosomal protein Dzip1l binds Cby, promotes ciliary bud formation, and acts redundantly with Bromi to regulate ciliogenesis in the mouse.Development2018,145, dev164236.
24．Zhang L, Yang H, Zhang W, Liang Z, Huang Q,Xu G, Zhen X*, Zheng LT*. Clk1-regulated aerobic glycolysis is involved in glioma chemoresistance.J. Neurochem.2017,142, 574-588.
25．Zhou Y, Xiong L, Zhang Y, Yu R, Jiang X,Xu G*. Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.J. Proteomics2016,148, 94-104.
26．Duan W, Chen S, Zhang Y, Li D, Wang R, Chen S, Li J, Qiu X,Xu G*. Protein C-terminal enzymatic labeling identifies novel caspase cleavages during the apoptosis of multiple myeloma cells induced by kinase inhibition.Proteomics2016,16, 60-69.
27．Liu Y, Huang X, He X, Zhou Y, Jiang X, Chen-Kiang S, Jaffrey SR,*Xu G*. A novel effect of thalidomide and its analogs: suppression of cereblon ubiquitination enhances ubiquitin ligase function.FASEB J.2015,29, 4829-4839.
28．Deglincerti A, Liu Y, Colak D, Hengst U,Xu G*,Jaffrey SR*. Coupled local translation and degradation regulate growth cone collapse.Nat. Commun.2015,6, 6888.
29．Xu G*, Jiang X, Jaffrey SR*. A mental retardation-linked nonsense mutation in cereblon is rescued by proteasome inhibition.J. Biol. Chem.2013,288, 29573-29585.
30．Yoon Y, McKenna MC, Rollins DA, Song M, Nuriel T, Gross SS,Xu G, Glatt CE*. Anxiety-associated alternative polyadenylation of the serotonin transporter mRNA confers translational regulation by hnRNPK.Proc. Natl. Acad. Sci. U.S.A.2013,110, 11624-11629.
31．Xu G,Jaffrey SR*. The new landscape of protein ubiquitination.Nat. Biotechnol.(News & Views)2011,29, 1098-1100.
32．Xu G,Shin SY, Jaffrey SR*. Chemoenzymatic labeling of protein C-termini for positive selection of C-terminal peptides.ACS Chem. Biol.2011,6, 1015-1020.
33．Xu G,Paige JS, Jaffrey SR*. Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.Nat. Biotechnol.2010,28, 868-873.
